Fig. 2. Wip1 activates p38 and dephosphorylates p53 in trophoblasts under conditions of hypoxia. A: Lentiviral transfection efficiency was examined by FCM and IF. Scale bar 400 µm; B: Wip1 protein expression was confirmed by western blotting, n=4, *P<0.05 vs. scrambled shRNA, one-way ANOVA, Dunnett-t multiple comparison test; C-D. Representative Western blots of p-p38^{Thr180/Tyr182}, p38, cl-cas9, Mdm2, p-p53^Ser15, and p53 in HTR8/SVneo cells after 24 h of HII (A) or SIB (B) in the presence of 20 µM GSK2830371 (GSK), DMSO (0.1%), shWip1, or scrambled shRNA (MOI=50). n=4, (*P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, one-way ANOVA, Sidak's multiple comparisons test. Experiments were performed in triplicate.